Anti-CYB5R3 antibody [EPR3868] (ab133247)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3868] to CYB5R3
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CYB5R3 antibody [EPR3868]
See all CYB5R3 primary antibodies -
Description
Rabbit monoclonal [EPR3868] to CYB5R3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
A synthetic peptide corresponding to residues in human CYB5R3 (P00387).
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Positive control
- A431; Fetal liver; HeLa and U87 MG lysates. human testis tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3868 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CYB5R3 antibody [EPR3868] (ab133247) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Fetal liver cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : U87 MG cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
Note: Primary antibody ab133247 is diluted with 1% BSA -
Immunohistochemical analysis of paraffin embedded Human testis tissue labelling CYB5R3 with ab133247 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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