Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-XLF antibody [EPR15882-42] (ab189520)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15882-42] to XLF
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-XLF antibody [EPR15882-42]
    See all XLF primary antibodies

  • Description

    Rabbit monoclonal [EPR15882-42] to XLF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • NCCIT, 293, HepG2 and Jurkat cell lysates; A431 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15882-42

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-XLF antibody [EPR15882-42] (ab189520)
    Western blot - Anti-XLF antibody [EPR15882-42] (ab189520)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: XLF knockout HAP1 cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green – ab189520 observed at 38 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab189520 was shown to specifically react with XLF when XLF knockout samples were used. Wild-type and XLF knockout samples were subjected to SDS-PAGE. ab189520 and ab18058 (loading control to Vinculin) were both diluted at 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-42] (ab189520)
    Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-42] (ab189520)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling XLF with ab189520 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

  • Western blot - Anti-XLF antibody [EPR15882-42] (ab189520)
    Western blot - Anti-XLF antibody [EPR15882-42] (ab189520)
    All lanes : Anti-XLF antibody [EPR15882-42] (ab189520) at 1/20000 dilution

    Lane 1 : NCCIT cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • Anti-XLF antibody [EPR15882-42] (ab189520)
    Anti-XLF antibody [EPR15882-42] (ab189520)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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