Antibodies, Proteins, Kits and Reagents for Life Science

Anti-XLF antibody [EPR15882-42] (ab189520)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15882-42] to XLF
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-XLF antibody [EPR15882-42]
See all XLF primary antibodies
Description

Rabbit monoclonal [EPR15882-42] to XLF
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- NCCIT, 293, HepG2 and Jurkat cell lysates; A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15882-42
Isotype

IgG
Research areas

Western blot - Anti-XLF antibody [EPR15882-42] (ab189520)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: XLF knockout HAP1 cell lysate (20 µg)

Lanes 1 - 2: Merged signal (red and green). Green – ab189520 observed at 38 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab189520 was shown to specifically react with XLF when XLF knockout samples were used. Wild-type and XLF knockout samples were subjected to SDS-PAGE. ab189520 and ab18058 (loading control to Vinculin) were both diluted at 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-42] (ab189520)

Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling XLF with ab189520 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
Western blot - Anti-XLF antibody [EPR15882-42] (ab189520)

All lanes : Anti-XLF antibody [EPR15882-42] (ab189520) at 1/20000 dilution

Lane 1 : NCCIT cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 33 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?
Anti-XLF antibody [EPR15882-42] (ab189520)

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