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Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15882-36] to XLF - C-terminal
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-XLF antibody [EPR15882-36] - C-terminal
    See all XLF primary antibodies

  • Description

    Rabbit monoclonal [EPR15882-36] to XLF - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos, Jurkat and HepG2 whole cell lysate (ab7900); Human endometrial adenocarcinoma; HepG2 and NCCIT cells; Ramos cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15882-36

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)
    Western blot - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: XLF knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab189917 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab189917 was shown to specifically react with XLF in wild type cells as signal was lost in XLF knockout cells. Wild-type and XLF knockout samples were subjected to SDS-PAGE. ab189917 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)
    Flow Cytometry - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)

    Flow Cytometrical analysis of Ramos cells labeling XLF with ab189917 at 1/60 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)
    Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)

    Immunofluorescent analysis of paraformaldehyde-fixed NCCIT cells labeling XLF with ab189917 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).

  • Western blot - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)
    Western blot - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)
    All lanes : Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917) at 1/10000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)
    Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)

    Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling XLF with ab189917 at 1/250,  Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling XLF with ab189917 at 1/250 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)
    Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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