Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15882-36] to XLF - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-XLF antibody [EPR15882-36] - BSA and Azide free
    See all XLF primary antibodies

  • Description

    Rabbit monoclonal [EPR15882-36] to XLF - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild type HAP1 whole cell lysate; HeLa whole cell lysate.

  • General notes

    Ab232587 is the carrier-free version of ab189917. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232587 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)
    Western blot - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: XLF knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab189917 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab189917 was shown to specifically react with XLF in wild type cells as signal was lost in XLF knockout cells. Wild-type and XLF knockout samples were subjected to SDS-PAGE. ab189917 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189917).

  • Flow Cytometry - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)
    Flow Cytometry - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)

    Flow Cytometrical analysis of Ramos cells labeling XLF with ab189917 at 1/60 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189917).

  • Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)
    Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)

    Immunofluorescent analysis of paraformaldehyde-fixed NCCIT cells labeling XLF with ab189917 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189917).

  • Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)
    Immunocytochemistry/ Immunofluorescence - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)

    Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling XLF with ab189917 at 1/250,  Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189917).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling XLF with ab189917 at 1/250 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189917).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling XLF with ab189917 at 1/250 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189917).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)
    Anti-XLF antibody [EPR15882-36] - BSA and Azide free (ab232587)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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