Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

Key features and details

  • Mouse monoclonal [OTI1F4] to Glutamine Synthetase - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Dog, Human, African green monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free
    See all Glutamine Synthetase primary antibodies

  • Description

    Mouse monoclonal [OTI1F4] to Glutamine Synthetase - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Dog, Human, African green monkey

  • Immunogen

    Recombinant full length protein corresponding to Human Glutamine Synthetase aa 1-400.
    Database link: P15104

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, HT29, pCMV6-ENTRY GLUL-transfected HEK-293T cell lysate, IHC-P: Human breast, ovary, endometrium tissue; IF: HepG2, COS-7 whole cells, Flow: Jurkat, HeLa whole cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

    Immunohistochemical analysis of human endometrum tissue staining Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    All lanes : Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 7 : MDCK (canine kidney cell line) whole cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 42 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

    Immunofluorescence analysis of HepG2 (human liver hepatocellular carcinoma cell line) whole cell labeling Glutamine Synthetase with a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

    Immunohistochemical analysis of human breast tissue staining Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Flow Cytometry - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Flow Cytometry - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

    Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody. (Red) compared to a nonspecific negative control
    antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    All lanes : Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

    Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : pCMV6-ENTRY GLUL-transfected HEK-293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 42 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

    Immunofluorescence analysis of COS-7 (african green monkey kidney fibroblast-like cell line) whole cell labeling Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody..

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Flow Cytometry - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Flow Cytometry - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell using using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody. (Red) compared to a nonspecific negative control antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

    Immunohistochemical analysis of human ovary tissue staining Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

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