Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)
![Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)](https://www.abcam.com/ps/products/249/ab249408/Images/ab249408-449219-recombinant-anti-slc25a13citrin-antibody-epr9969b-western-blot-hela-lysates.jpg "Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free
See all SLC25A13/Citrin primary antibodies -
Description
Rabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HAP1 and SH-5YSY cell lysates.
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General notes
ab249408 is the carrier-free version of ab167166 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249408 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as SLC25A13
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9969(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SLC25A13 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab167166).
Lanes 1- 2: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab167166 was shown to react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265668 (knockout cell lysate ab258192) was used. Wild-type HeLa and SLC25A13 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)](https://www.abcam.com/ps/products/249/ab249408/Images/ab249408-449544-anti-slc25a13-antibody-epr9969b-western-blot-hap1-sh5ysy.jpg)
Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/500 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SLC25A13 knockout HAP1 cell lysate
Lane 3 : SH-5YSY cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 70 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab167166).
Lanes 1 - 3: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab167166 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. ab167166 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)](https://www.abcam.com/ps/products/249/ab249408/Images/ab249408-2-benefits-of-recombinant-antibodies.png)
Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)
