Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free
    See all SLC25A13/Citrin primary antibodies

  • Description

    Rabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HAP1 and SH-5YSY cell lysates.

  • General notes

    ab249408 is the carrier-free version of ab167166 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249408 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SLC25A13

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)
    Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)
    All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SLC25A13 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab167166).

      Lanes 1- 2: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab167166 was shown to react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265668 (knockout cell lysate ab258192) was used. Wild-type HeLa and SLC25A13 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)
    Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)
    All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/500 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : SLC25A13 knockout HAP1 cell lysate
    Lane 3 : SH-5YSY cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 70 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab167166).

    Lanes 1 - 3    : Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab167166 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. ab167166 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)
    Anti-SLC25A13/Citrin antibody [EPR9969(B)] - BSA and Azide free (ab249408)

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