Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Key features and details

  • Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Dog, Human, African green monkey, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free
    See all Tristetraprolin/TTP primary antibodies

  • Description

    Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Dog, Human, African green monkey, Recombinant fragment

  • Immunogen

    Recombinant full length protein corresponding to Human Tristetraprolin/TTP aa 1-350.
    Database link: P26651

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, SVT2, A549, COS7, Jurkat, MDCK and MCF7 cells lysate; IHC-P: Human colon, colon adenocarcinoma, ovary, endometrium, prostate tissue; IF: COS7 cell lysate; Flow: HEK-293T transfected with Tristetraprolin

  • General notes

    ab273646 is the carrier-free version of ab124024. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Immunocytochemistry/ Immunofluorescence - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

    Immunofluorescence analysis of transiently pCMV6-ENTRY ZFP36-transfected COS7 (african green monkey kidney fibroblast-like cell line) cells labeling TTP using ab124024 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

    Immunohistochemical analysis of paraffin-embedded human colon tissue staining TTP using ab124024 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

  • Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646) at 1/200 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : SV-T2 (Simian Virus 40-transformed BALB/c mouse embryo fibroblasts cell line) whole cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 4 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 6 : MDCK (canine kidney cell line) whole cell lysate
    Lane 7 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 34 kDa

  • Flow Cytometry - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Flow Cytometry - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

    HEK-293T cells transfected with either overexpressed plasmid (Red) or empty vector control plasmid (Blue) were immunostained by
    ab124024, and then analyzed by flow cytometry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

  • Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646) at 1/1000 dilution

    Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY ZFP36-transfected HEK-293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

    Immunohistochemical analysis of paraffin-embedded human adenocarcinoma colon tissue staining TTP using ab124024 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue staining TTP using ab124024 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue staining TTP using ab124024 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

    Immunohistochemical analysis of paraffin-embedded human prostate tissue staining TTP using ab124024 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

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