Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Key features and details

Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP - BSA and Azide free
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Dog, Human, African green monkey, Recombinant fragment
Isotype: IgG1

Product name

Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free
See all Tristetraprolin/TTP primary antibodies
Description

Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Dog, Human, African green monkey, Recombinant fragment
Immunogen

Recombinant full length protein corresponding to Human Tristetraprolin/TTP aa 1-350.
Database link: P26651

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Positive control
- WB: HeLa, SVT2, A549, COS7, Jurkat, MDCK and MCF7 cells lysate; IHC-P: Human colon, colon adenocarcinoma, ovary, endometrium, prostate tissue; IF: COS7 cell lysate; Flow: HEK-293T transfected with Tristetraprolin
General notes

ab273646 is the carrier-free version of ab124024. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.3
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

OTI8B5
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Immunofluorescence analysis of transiently pCMV6-ENTRY ZFP36-transfected COS7 (african green monkey kidney fibroblast-like cell line) cells labeling TTP using ab124024 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Immunohistochemical analysis of paraffin-embedded human colon tissue staining TTP using ab124024 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).
Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646) at 1/200 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : SV-T2 (Simian Virus 40-transformed BALB/c mouse embryo fibroblasts cell line) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : MDCK (canine kidney cell line) whole cell lysate
Lane 7 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 34 kDa
Flow Cytometry - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

HEK-293T cells transfected with either overexpressed plasmid (Red) or empty vector control plasmid (Blue) were immunostained by
ab124024, and then analyzed by flow cytometry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).
Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646) at 1/1000 dilution

Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY ZFP36-transfected HEK-293T cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma colon tissue staining TTP using ab124024 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Immunohistochemical analysis of paraffin-embedded human ovary tissue staining TTP using ab124024 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue staining TTP using ab124024 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Immunohistochemical analysis of paraffin-embedded human prostate tissue staining TTP using ab124024 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

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