Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-iNOS antibody [EPR16630] (ab205529)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16630] to iNOS
  • Suitable for: IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-iNOS antibody [EPR16630]
    See all iNOS primary antibodies

  • Description

    Rabbit monoclonal [EPR16630] to iNOS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 treated with 0.1 µg/ml LPS for 6 hours whole cell lysate. IP: RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-iNOS antibody [EPR16630] (ab205529)
    Western blot - Anti-iNOS antibody [EPR16630] (ab205529)
    All lanes : Anti-iNOS antibody [EPR16630] (ab205529) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 µg/ml LPS for 6 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 131 kDa
    Observed band size: 131 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-iNOS antibody [EPR16630] (ab205529)
    Immunoprecipitation - Anti-iNOS antibody [EPR16630] (ab205529)

    iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 mg/ml LPS for 6 hours whole cell extract with ab205529 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab205529 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract 10ug (Input).

    Lane 2: ab205529 IP in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205529 in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.

     

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

  • Anti-iNOS antibody [EPR16630] (ab205529)
    Anti-iNOS antibody [EPR16630] (ab205529)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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