Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP16634] to iNOS
Suitable for: ELISA, WB
Reacts with: Mouse, Human, Recombinant fragment

Product name

Anti-iNOS antibody [EP16634]
See all iNOS primary antibodies
Description

Rabbit monoclonal [EP16634] to iNOS
Host species

Rabbit
Tested Applications & Species

Application Species
ELISA
Recombinant fragment

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell lysate; Human fetal brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ELISA	Recombinant fragment
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP16634
Isotype

IgG
Research areas

Western blot - Anti-iNOS antibody [EP16634] (ab202417)

All lanes : Anti-iNOS antibody [EP16634] (ab202417) at 1/10000 dilution

Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 mg/ml LPS for 6 hours whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 131 kDa
Observed band size: 131 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
ELISA - Anti-iNOS antibody [EP16634] (ab202417)

ELISA analysis of House mouse iNos2 recombinant protein at 1000 ng/mL with ab202417. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Western blot - Anti-iNOS antibody [EP16634] (ab202417)

Anti-iNOS antibody [EP16634] (ab202417) at 1/1000 dilution + Human fetal brain lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 131 kDa
Observed band size: 131 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-iNOS antibody [EP16634] (ab202417)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com