Antibodies, Proteins, Kits and Reagents for Life Science

371 894 ₸ Product size

100 µl 371 894 ₸

Order now and get it on Tuesday March 02, 2021

Anti-iNOS antibody [EPR16635] (ab178945)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16635] to iNOS
Suitable for: ELISA, WB, ICC/IF, IP
Reacts with: Mouse, Human

Product name

Anti-iNOS antibody [EPR16635]
See all iNOS primary antibodies
Description

Rabbit monoclonal [EPR16635] to iNOS
Host species

Rabbit
Specificity

This antibody shows low affinity on human samples.

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: RAW 264.7 treated with 0.1 µg/mL LPS for 6 hours, HepG2 treated with 10 µg/mL LPS for 6 hours,whole cell lysates; Human fetal brain lysate. ICC/IF: RAW 264.7 cells treated with LPS (0.1 µg/mL), for 6 hours. IP: RAW 264.7 whole cell lysate treated with 1µg/mL LPS for 24h.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16635
Isotype

IgG
Research areas

ELISA - Anti-iNOS antibody [EPR16635] (ab178945)

ELISA using ab178945 at varying antibody concentrations and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Western blot - Anti-iNOS antibody [EPR16635] (ab178945)

All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/20000 dilution

Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 whole cell lysate treated with 0.1 µg/mL LPS for 6 hours

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 131 kDa
Observed band size: 131 kDa

Exposure time: 30 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] (ab178945)

Immunocytochemistry/Immunofluorescence analysis of RAW 264.7 non-treated and LPS treated (0.1 µg/mL) cells labelling iNOS with ab178945 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, Alexa Fluor^® 594 conjugated anti-alpha tubulin (1/200). Nuclei counterstained with DAPI (blue).

Secondary antibody only controls performed on non-treated and treated cells.
Immunoprecipitation - Anti-iNOS antibody [EPR16635] (ab178945)

iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate treated with 1 µg/ml LPS for 24h with ab178945 at 1/100 dilution.

Lane 1: RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h,10ug (Input).

Lane 2: ab178945 IP in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab178945 in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h.

Western blot was performed from the immunoprecipitate using ab178945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Western blot - Anti-iNOS antibody [EPR16635] (ab178945)

All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/500 dilution

Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 10ug/ml lipopolysaccharides for 6 hours whole cell lysates
Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 4 : THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml lipopolysaccharides for 3 hours whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 131 kDa

Exposure time: 3 minutes
Anti-iNOS antibody [EPR16635] (ab178945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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