Anti-iNOS antibody (ab15323)
Key features and details
- Rabbit polyclonal to iNOS
- Suitable for: WB
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-iNOS antibody
See all iNOS primary antibodies -
Description
Rabbit polyclonal to iNOS -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody shares 84% homology with Rat iNOS. Ab15323 has been batch tested using mouse lysates/tissues only. Some customers have successfully used ab15323 with rat. Please contact Abcam Scientific Support for more information.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Recombinant fragment
Predicted to work with: Rat, Human
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Immunogen
Synthetic peptide corresponding to Mouse iNOS.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-iNOS antibody (ab15323)All lanes : Anti-iNOS antibody (ab15323) at 1 µg/ml
Lane 1 : Raw264.7
Lane 2 : Raw264.7 + LPS (1 ug per mL; 18 hours)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR- goat anti-rabbit (green) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDaThis blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with rabbit polyclonal to iNOS (ab15323; 1 ug x mL-1 (based on antibody concentration of 0.2mg/ml - lot GR126616-1) and the loading control mouse anti-GAPDH antibody (ab8245; 1:10000) overnight at 4°C. Antibody binding was detected using infrared (IR) labelled goat anti-rabbit (green; 1:10000) and IR-goat anti-mouse (red; 1:10000) for 1 hour at room temperature before imaging.
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Western blot - Anti-iNOS antibody (ab15323)All lanes : Anti-iNOS antibody (ab15323) at 5 µg/ml
Lane 1 : Raw 264.7 whole cell lysate + PMA
Lane 2 : Raw 264.7 whole cell lysate + PMA + LPS + Brefeldin A
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
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Western blot - Anti-iNOS antibody (ab15323)All lanes : Anti-iNOS antibody (ab15323) at 5 µg/ml
Lane 1 : Human recombinant iNOS protein
Lane 2 : Mouse recombinant iNOS protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Exposure time: 1 minuteMembrane was blocked in 2% milk for 1 hour at RT. Primary anitbody was incubated at 4°C overnight.
