Recombinant human PRMT5 + MEP50 protein (ab198151)
Key features and details
- Expression system: Mammalian
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PRMT5 + MEP50 protein
See all PRMT5 + MEP50 proteins and peptides -
Biological activity
Assay Conditions:
50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 10-100 ng methyltransferase PRMT5) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
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Purity
>= 75 % SDS-PAGE. -
Expression system
Mammalian -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
AAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFK REFIQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRR NSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMF WMRVPLVAPEDLRDDIIENAPTTHTEEYSGEEKTWMWWHNFRTLCDYSKR IAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQ RLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFA KGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPE EEKDTNVQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTL ENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLD GAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMP YVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGF AGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVR FWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL -
Predicted molecular weight
73 kDa including tags -
Amino acids
2 to 637 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
NM_006109, expressed in FreeStyle 293-F cells.
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Amino Acid Sequence 2
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Species
Human -
Sequence
RKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSG RCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSG AVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKV WDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPK PASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSS AVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVR DATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE -
Predicted molecular weight
38 kDa including tags -
Amino acids
2 to 342 -
Tags
His tag N-Terminus -
Additional sequence information
NM_024102, expressed in FreeStyle 293-F cells.
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Preparation and Storage
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Expressed in FreeStyle 293-F cells.
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Concentration information loading...
Images
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SDS-PAGE - Recombinant human PRMT5 + MEP50 protein (ab198151)
10% SDS-PAGE analysis of ab198151 (5μg).
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Functional Studies - Recombinant human PRMT5 + MEP50 protein (ab198151)Specific activity of ab198151.
