Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling iNOS with Purified ab115819 at 1/100 dilution (0.65 µg/ml).

Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6.0.

Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody.

Negative control: PBS instead of the primary antibody.

Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling iNOS with Purified ab115819 at 1/100 dilution (0.65 µg/ml).

Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6.0.

Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody.

Negative control: PBS instead of the primary antibody.

Hematoxylin was used as a counterstain.

Flow cytometry analysis of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells treated with 0.1ug/ml Lipopolysaccharides for 24hours labeling iNOS with purified ab115819 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

Formalin-fixed, paraffin-embedded human lung stained for iNOS using ab115819 at a dilution of 1/100 in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung stained for iNOS using ab115819 at a dilution of 1/100 in immunohistochemical analysis.