Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAP1A antibody [EPR18994] (ab184350)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18994] to MAP1A
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-MAP1A antibody [EPR18994]
    See all MAP1A primary antibodies

  • Description

    Rabbit monoclonal [EPR18994] to MAP1A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse embryo, brain and spinal cord lysates; Rat brain lysate; Neuro-2a and C6 whole cell lysates. ICC/IF: Neuro-2a and C6 cells. Flow Cyt: Neuro-2a cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] (ab184350)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] (ab184350)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184350 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Western blot - Anti-MAP1A antibody [EPR18994] (ab184350)
    Western blot - Anti-MAP1A antibody [EPR18994] (ab184350)
    All lanes : Anti-MAP1A antibody [EPR18994] (ab184350) at 1/5000 dilution

    Lane 1 : Mouse embryo lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Mouse spinal cord lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 305 kDa
    Observed band size: 26,300 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MAP1A antibody [EPR18994] (ab184350)
    Western blot - Anti-MAP1A antibody [EPR18994] (ab184350)
    Anti-MAP1A antibody [EPR18994] (ab184350) at 1/1000 dilution + Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 305 kDa
    Observed band size: 26,300 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MAP1A antibody [EPR18994] (ab184350)
    Western blot - Anti-MAP1A antibody [EPR18994] (ab184350)
    Anti-MAP1A antibody [EPR18994] (ab184350) at 1/2000 dilution + C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 305 kDa
    Observed band size: 300 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] (ab184350)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] (ab184350)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling MAP1A with ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
     -ve control 1: ab184350 at 1/1000 dilution followed by ab150120  at 1/1000 dilution.
     -ve control 2: ab7291 at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Flow Cytometry - Anti-MAP1A antibody [EPR18994] (ab184350)
    Flow Cytometry - Anti-MAP1A antibody [EPR18994] (ab184350)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with ab184350 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal  [EPR25A]- Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-MAP1A antibody [EPR18994] (ab184350)
    Anti-MAP1A antibody [EPR18994] (ab184350)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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