Anti-eNOS (phospho S1177) antibody (ab184154)
Key features and details
- Rabbit Polyclonal to eNOS (phospho S1177)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eNOS (phospho S1177) antibody
See all eNOS primary antibodies -
Description
Rabbit Polyclonal to eNOS (phospho S1177) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Pig
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Immunogen
Synthetic peptide within Human eNOS (phospho S1177). The exact sequence is proprietary.
Database link: P29474 -
Positive control
- THP1 whole cell lysate (untreated and treated with 100 nM PMA treatment for 6, 24 or 48 hr), Jurkat, Raji and NCI-H929 whole cell lysate; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)All lanes : Anti-eNOS (phospho S1177) antibody (ab184154) at 1/1000 dilution
Lane 1 : HUVEC whole cell lysate
Lane 2 : VEGF (ab9571) treated HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit antibody (green) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 133 kDa
Additional bands at: 150 kDa (possible post-translational modification)2e7 HUVEC, grown in serum free medium for 18 hours, were harvested with 0.5 mM EDTA. Cells, collected and resuspended in 10 mL cell culture medium, were stimulated for 2 minutes with 50 ng per mL VEGF (ab9571), after which 30 mL of ice-cold PBS was added. The tubes were spun for 2 min at 1.5k RPM and cell pellets were snap frozen. Control cells were treated in parallel, but without the addition of VEGF.
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour before being incubated with anti-eNOS (phospho S1177) antibody (ab184154; diluted 1:1000) and loading control (anti-GAPDH antibody [6C5] ab8245; diluted 1:20000) at 4°C overnight. Antibody binding was detected using infrared labelled goat anti-rabbit (green) or anti-mouse (red) antibodies (diluted 1:20000) for 1 hour at room temperature before imaging.
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Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)All lanes : Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lanes 1 & 3 & 5 : THP1 whole cell lysate
Lane 2 : THP1 whole cell lysate (100 nM PMA treatment for 6 hr)
Lane 4 : THP1 whole cell lysate (100 nM PMA treatment for 24 hr)
Lane 6 : THP1 whole cell lysate (100 nM PMA treatment for 48 hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 133 kDa5 % SDS-PAGE
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Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)All lanes : Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 133 kDa5 % SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-eNOS (phospho S1177) antibody (ab184154)Immunofluorescent analysis of methanol fixed HeLa cells labeling eNOS using ab184154 at 1/500 dilution. Image at the right costained with Hoechst 33342.
