Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20991] to eNOS (phospho S1177) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free
    See all eNOS primary antibodies

  • Description

    Rabbit monoclonal [EPR20991] to eNOS (phospho S1177) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Dot blot, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC +/- VEGF, whole cell lysate.

  • General notes

    Ab230158 is the carrier-free version of ab215717. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230158 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)
    Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)
    All lanes : Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) at 1/1000 dilution

    Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell line) treated with 10 ng/ml VEGF for 5 min, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 133 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The 60 kDa band may be a cleaved form of eNOS (PMID: 16195740).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215717).

  • Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)
    Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)

    Dot blot analysis of eNOS (phospho S1177) labeled with ab215717 at 1/1000 dilution.

    Lane 1: eNOS (phospho S1177) peptide.
    Lane 2: eNOS non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Exposure time: 1 minute.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215717).

  • Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)
    Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)

    eNOS (phospho S1177) was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) treated with 10 ng/ml VEGF for 5 min, whole cell lysate with ab215717 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215717 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HUVEC treated with VEGF whole cell lysate 10 μg (Input).
    Lane 2: ab215717 IP in HUVEC treated with VEGF whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215717 in HUVEC treated with VEGF whole cell lysate.

    Exposure time: 30 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    The 60 kDa band may be a cleaved form of eNOS (PMID: 16195740).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215717).

  • Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)
    Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (ab230158)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.