Anti-eNOS antibody [6H2] (ab91205)
Key features and details
- Mouse monoclonal [6H2] to eNOS
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-eNOS antibody [6H2]
See all eNOS primary antibodies -
Description
Mouse monoclonal [6H2] to eNOS -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified recombinant fragment of Human eNOS expressed in E. coli.
-
Positive control
- WB: HuVEC Human lymph node, colon cancer, stomach cancer, and ovary cancer.
-
General notes
This product was changed from ascites to supernatant. Lot no’s high than GR129588-16 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6H2 -
Isotype
IgG1 -
Research areas
Images
-
Overlay histogram showing HEK293 cells stained with ab91205 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91205, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
-
ab91205, at a 1/200 dilution, staining eNOS in paraffin embedded Human lymph node (left) and colon cancer (right) tissues by Immunohistochemistry. Detection was by DAB staining.
-
ab91205, at a 1/200 dilution, staining eNOS in paraffin embedded Human stomach cancer (left) and ovary cancer (right) tissues by Immunohistochemistry. Detection was by DAB staining.