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Neuroscience Neurotransmission Nitric Oxide NOS

Anti-eNOS antibody [EPR19296] (ab199956)

Price and availability

304 886 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-eNOS antibody [EPR19296] (ab199956)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19296] to eNOS
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-eNOS antibody [EPR19296]
    See all eNOS primary antibodies
  • Description

    Rabbit monoclonal [EPR19296] to eNOS
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal heart, fetal kidney and fetal liver lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, heart and spleen lysates; whole cell lysate from HepG2 cells treated with 10 µg/ml LPS for 2 hours and 6 hours; DDDDK tagged human eNOS full length recombinant protein (aa2-1203) ab198066. IP: HepG2 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19296
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Cardiovascular
    • Blood
    • Platelets
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Nitric oxide associated
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Images

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    All lanes : Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID: 15210449 & 15936740).

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    All lanes : Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2, 3, 4, 6 and 7: 30 seconds; Lane 5: 2 minutes.

    eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID: 15210449 & 15936740).

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    All lanes : Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Exposure Time: Lane1: 40 seconds; Lane2: 180 seconds

    The expression profile observed in HeLa is consistent with the literature (PMID: 19925457).
    Negative control: HeLa (PMID: 19925457)

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    All lanes : Anti-eNOS antibody [EPR19296] (ab199956) at 1/5000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : Whole cell lysate from HepG2 cells treated with 10 µg/ml LPS for 2 hours
    Lane 3 : Whole cell lysate from HepG2 cells treated with 10 µg/ml LPS for 6 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:17855661 & 11004215).

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
    All lanes : Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

    Lane 1 : Recombinant Human eNOS protein (Tagged) (ab198066) at 0.01 µg
    Lane 2 : Recombinant Human iNOS protein (ab135010) at 0.05 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

     

  • Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)
    Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)

    eNOS was immunoprecipitated from 1 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199956 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199956 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (Input).

    Lane 2: ab199956 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199956 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-eNOS antibody [EPR19296] (ab199956)
    Anti-eNOS antibody [EPR19296] (ab199956)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-eNOS antibody [EPR19296] (ab199956)

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