Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

1 pack 308 236 ₸

Order now and get it on Tuesday March 09, 2021

Parkinson's Disease Research Antibody Panel (ab226767)

Product name

Parkinson's Disease Research Antibody Panel
Product overview

ab226767 is a Parkinson's Disease research panel containing 5 recombinant rabbit monoclonal antibodies and 1 Anti-Rabbit IgG (HRP) secondary antibody: Anti-alpha synuclein antibody, Anti-LRRK2 antibody, Anti-PINK1 antibody, Anti-PARK/DJ1 antibody and Goat Anti-Rabbit IgG (H&L) (HRP).

The hallmark pathologies of Parkinson’s disease include the loss of dopaminergic neurons from the pars compacta, a portion of the substantia nigra in the midbrain, causing slowness of movement (bradykinesia), resting tremor and rigidity. This is coupled with the presence of intracellular protein aggregates known as Lewy bodies.

The antibodies in this panel were selected for their exceptional performance in imaging applications, alongside other applications. Please see the individual datasheets for additional information.
Notes

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Directly conjugated versions of our antibodies are available and ready to use for multicolor analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Storage instructions

Store at -20°C. Please refer to protocols.

Components	1 packs
ab138501 - Anti-alpha Synuclein antibody [MJFR1]	1 x 10µl
ab133474 - Anti-LRRK2 antibody [MJFF2 (c41-2)]	1 x 10µl
ab76008 - Anti-PARK7/DJ1 antibody [EP2815Y]	1 x 10µl
ab216144 - Anti-PINK1 antibody [EPR20730]	1 x 10µl
ab205718 - Goat Anti-Rabbit IgG H+L (HRP)	1 x 100µg

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)

ab76008, at 1/250 dilution, staining PARK7/DJ1 in human brain by immunohistochemistry using paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR20730] (ab216144)

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma)(+/- treatment with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours) cells labeling PINK1 with ab216144 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells treated with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

The negative controls are as follows:
-ve control: PBS, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab133474)

Immunohistochemical analysis of mouse brain tissue, staining LRRK2 with ab133474.

Antigen retrieval was performed by heat mediation and tissue was blocked with 2% goat serum. Sections were incubated with primary antibody (1/200) overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

IHC image of alpha Synuclein staining in normal Human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Parkinson's Disease Research Antibody Panel (ab226767)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com