Anti-MRP2 antibody [M2 III-6] (ab3373)
![Anti-MRP2 antibody [M2 III-6] (ab3373)](https://www.abcam.com/ps/products/3/ab3373/Images/ab3373-353434-anti-mrp2-antibody-m2-iii-6-western-blot-knock-out-a549-abcc2-hepg2-human-lysates.jpg "Anti-MRP2 antibody [M2 III-6] (ab3373)")
Key features and details
- Mouse monoclonal [M2 III-6] to MRP2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-MRP2 antibody [M2 III-6]
See all MRP2 primary antibodies -
Description
Mouse monoclonal [M2 III-6] to MRP2 -
Host species
Mouse -
Specificity
This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Fusion protein corresponding to Human MRP2 aa 1339-1541 (C terminal).
-
Epitope
This antibody reacts with an internal epitope of MRP 2. -
General notes
We received mixed feedback about rat species so we are removing this from tested species list for now. The immunogen is 84% homologous to human protein so theoretically it should cross react however considering the latest customer feedback we no longer guarantee this species.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Constituent: 0.7% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
M2 III-6 -
Isotype
IgG2a -
Research areas
Images
-
Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)All lanes : Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/20 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : ABCC2 knockout A549 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 174 kDaLanes 1 - 3: Merged signal (red and green). Green - ab3373 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab3373 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab3373 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)](https://www.abcam.com/ps/products/3/ab3373/Images/ab3373-349941-anti-mrp2-antibody-m2-III-6-western-blot-human.jpg)
Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373) This image is courtesy of an anonymous AbreviewAnti-MRP2 antibody [M2 III-6] (ab3373) at 1/500 dilution + WIF-B cells, the hepatoma-derived hybrid cell line at 30 µg with 5% milk in PBST
Secondary
HRP conjugated Donkey anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 174 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
