Anti-MRP2 antibody [M2III-5] (ab15603)
![Anti-MRP2 antibody [M2III-5] (ab15603)](https://www.abcam.com/ps/products/15/ab15603/Images/ab15603-353437-anti-mrp2-antibody-m2iii-5-western-blot-knock-out-a549-abcc2-human-lysates.jpg "Anti-MRP2 antibody [M2III-5] (ab15603)")
Key features and details
- Mouse monoclonal [M2III-5] to MRP2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-MRP2 antibody [M2III-5]
See all MRP2 primary antibodies -
Description
Mouse monoclonal [M2III-5] to MRP2 -
Host species
Mouse -
Specificity
This antibody does not cross-react with the human MDR1 P-gp, MRP1, MRP3, or MRP5 gene products. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This antibody was raised against a fusion protein of the bacterial maltose binding protein and rat MRP2, containing the 202-amino acid C terminal end of the transporter protein.
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Epitope
ab15603 reacts with an internal epitope of MRP2; a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: Tissue culture supernatant, 0.7% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
M2III-5 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-MRP2 antibody [M2III-5] (ab15603)All lanes : Anti-MRP2 antibody [M2III-5] (ab15603) at 1/20 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : ABCC2 knockout A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 185.4 kDaLanes 1 - 2: Merged signal (red and green). Green - ab15603 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab15603 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab15603 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
