Anti-BTK antibody [10D11] (ab254141)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [10D11] to BTK
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-BTK antibody [10D11]
See all BTK primary antibodies -
Description
Mouse monoclonal [10D11] to BTK -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji, Ramos, K562 and NAMALWA whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
10D11 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-BTK antibody [10D11] (ab254141) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 3 : NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 6 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 114 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Negative control: Jurkat, MOLT-4 (PMID 24759210).
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