Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MLD antibody [EPR9681] (ab167169)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9681] to MLD
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MLD antibody [EPR9681]
    See all MLD primary antibodies

  • Description

    Rabbit monoclonal [EPR9681] to MLD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MLD (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: 293T, T47D and HepG2 whole cell lysate (ab7900). IHC-P: Human breast carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as DEGS1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MLD antibody [EPR9681] (ab167169)
    Western blot - Anti-MLD antibody [EPR9681] (ab167169)
    All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab167169 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab167169 Anti-MLD antibody [EPR9681]  was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab167169 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MLD with ab167169 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-MLD antibody [EPR9681] (ab167169)
    Western blot - Anti-MLD antibody [EPR9681] (ab167169)
    All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : T47D cell lysates
    Lane 3 : HepG2 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 38 kDa

  • Anti-MLD antibody [EPR9681] (ab167169)
    Anti-MLD antibody [EPR9681] (ab167169)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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