Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9681] to MLD
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-MLD antibody [EPR9681]
See all MLD primary antibodies
Description

Rabbit monoclonal [EPR9681] to MLD
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MLD (C terminal). The exact sequence is proprietary.
Positive control
- WB: 293T, T47D and HepG2 whole cell lysate (ab7900). IHC-P: Human breast carcinoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as DEGS1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9681
Isotype

IgG
Research areas

Western blot - Anti-MLD antibody [EPR9681] (ab167169)

All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 38 kDa
Observed band size: 38 kDa

Lanes 1-4: Merged signal (red and green). Green - ab167169 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.

ab167169 Anti-MLD antibody [EPR9681] was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab167169 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MLD with ab167169 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-MLD antibody [EPR9681] (ab167169)

All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

Lane 1 : 293T cell lysates
Lane 2 : T47D cell lysates
Lane 3 : HepG2 cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa
Anti-MLD antibody [EPR9681] (ab167169)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com