Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MLD antibody [EPR9680] (ab185237)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9680] to MLD
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MLD antibody [EPR9680]
    See all MLD primary antibodies

  • Description

    Rabbit monoclonal [EPR9680] to MLD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, U87-MG, 293 and T47D cell lysates.

  • General notes

     

     

     This product was previously labelled as DEGS1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MLD antibody [EPR9680] (ab185237)
    Western blot - Anti-MLD antibody [EPR9680] (ab185237)
    All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab185237 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab185237 Anti-MLD antibody [EPR9680] was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab185237 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MLD antibody [EPR9680] (ab185237)
    Western blot - Anti-MLD antibody [EPR9680] (ab185237)
    All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/10000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

  • Western blot - Anti-MLD antibody [EPR9680] (ab185237)
    Western blot - Anti-MLD antibody [EPR9680] (ab185237)
    All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : T47D cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

  • Anti-MLD antibody [EPR9680] (ab185237)
    Anti-MLD antibody [EPR9680] (ab185237)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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