Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µg 321 638 ₸ 10 µg 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [K56A/50] to CASK
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-CASK antibody [K56A/50]
See all CASK primary antibodies
Description

Mouse monoclonal [K56A/50] to CASK
Host species

Mouse

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain lysate; mouse brain lysate; rat brain lysate; mouse cerebellum lysate; rat cerebellum lysate. IHC-P: Rat liver, stomach and pancreas tissue; mouse liver tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

K56A/50
Isotype

IgG1
Research areas

Western blot - Anti-CASK antibody [K56A/50] (ab252540)

All lanes : Anti-CASK antibody [K56A/50] (ab252540) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse cerebellum lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat cerebellum lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Anti-mouse IgG for IP (HRP) (ab131368) at 1/100000 dilution
Lanes 2-5 : Peroxidase-Conjugated Goat anti-Mouse IgG(H+L) at 1/100000 dilution

Predicted band size: 105 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 125 seconds; Lanes 2-5: 26 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID: 15236336).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] (ab252540)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CASK with ab252540 at 0.134µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on mouse liver is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] (ab252540)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CASK with ab252540 at 0.134µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous and cytoplasmic staining on rat stomach is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] (ab252540)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling CASK with ab252540 at 0.134µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on rat pancreas is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] (ab252540)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CASK with ab252540 at 0.134µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on rat liver is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.
Anti-CASK antibody [K56A/50] (ab252540)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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