Anti-NOL5A antibody - C-terminal (ab229497)
Key features and details
- Rabbit polyclonal to NOL5A - C-terminal
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOL5A antibody - C-terminal
See all NOL5A primary antibodies -
Description
Rabbit polyclonal to NOL5A - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment within Human NOL5A (C terminal). The exact sequence is proprietary.
Database link: O00567 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as NOL5A
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NOL5A antibody - C-terminal (ab229497)All lanes : Anti-NOL5A antibody - C-terminal (ab229497) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 66 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody - C-terminal (ab229497)Paraffin-embedded human breast carcinoma tissue stained for NOL5A using ab229497 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody - C-terminal (ab229497)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NOL5A (green) using ab229497 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
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Immunoprecipitation - Anti-NOL5A antibody - C-terminal (ab229497)NOL5A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229497. Western blot was performed from the immunoprecipitate using ab229497. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab229497 in HEK-293T whole cell extract.
Lane 2: ab229497 IP in HEK-293T whole cell extract.
