Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABCB5 antibody [5H3C6] (ab140667)

Key features and details

  • Mouse monoclonal [5H3C6] to ABCB5
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, ELISA
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-ABCB5 antibody [5H3C6]
    See all ABCB5 primary antibodies

  • Description

    Mouse monoclonal [5H3C6] to ABCB5

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Purified recombinant fragment, corresponding to amino acids 481-674 of Human ABCB5, expressed in E. Coli.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human ABCB5 protein; A431 cell lysate; HepG2 cells; Human liver cancer and breast cancer tissues.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR206345-20 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabilizer.
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    5H3C6

  • Isotype

    IgG1

  • Research areas

Images

  • Flow Cytometry - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Flow Cytometry - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Flow cytometric analysis of HepG2 cells labeling ABCB5 with ab140667 at 1/200 dilution (green);negative control (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Immunofluorescent analysis of HepG2 cells labeling ABCB5 with ab140667 at 1/200 dilution (green); Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Western blot - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Anti-ABCB5 antibody [5H3C6] (ab140667) at 1/500 dilution + A431 cell lysate.

    Predicted band size: 90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling ABCB5 with ab140667 at 1/200 dilution followed by DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB5 antibody [5H3C6] (ab140667)
    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ABCB5 with ab140667 at 1/200 dilution followed by DAB staining.
  • ELISA - Anti-ABCB5 antibody [5H3C6] (ab140667)
    ELISA - Anti-ABCB5 antibody [5H3C6] (ab140667)

    ELISA analysis using ab140667. Antigen = Purified recombinant fragment, corresponding to amino acids 481-674 of Human ABCB5, expressed in E. Coli.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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