Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µl 274 732 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Mouse monoclonal [5H3C6] to ABCB5
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, ELISA
Reacts with: Human, Recombinant fragment
Isotype: IgG1

Product name

Anti-ABCB5 antibody [5H3C6]
See all ABCB5 primary antibodies
Description

Mouse monoclonal [5H3C6] to ABCB5
Host species

Mouse

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Purified recombinant fragment, corresponding to amino acids 481-674 of Human ABCB5, expressed in E. Coli.

Run BLAST with Run BLAST with
Positive control
- Recombinant Human ABCB5 protein; A431 cell lysate; HepG2 cells; Human liver cancer and breast cancer tissues.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR206345-20 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS

Contains 0.5% protein stabilizer.
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

5H3C6
Isotype

IgG1
Research areas

Flow Cytometry - Anti-ABCB5 antibody [5H3C6] (ab140667)

Flow cytometric analysis of HepG2 cells labeling ABCB5 with ab140667 at 1/200 dilution (green);negative control (red).
Immunocytochemistry/ Immunofluorescence - Anti-ABCB5 antibody [5H3C6] (ab140667)

Immunofluorescent analysis of HepG2 cells labeling ABCB5 with ab140667 at 1/200 dilution (green); Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot - Anti-ABCB5 antibody [5H3C6] (ab140667)

Anti-ABCB5 antibody [5H3C6] (ab140667) at 1/500 dilution + A431 cell lysate.

Predicted band size: 90 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB5 antibody [5H3C6] (ab140667)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling ABCB5 with ab140667 at 1/200 dilution followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB5 antibody [5H3C6] (ab140667)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ABCB5 with ab140667 at 1/200 dilution followed by DAB staining.
ELISA - Anti-ABCB5 antibody [5H3C6] (ab140667)

ELISA analysis using ab140667. Antigen = Purified recombinant fragment, corresponding to amino acids 481-674 of Human ABCB5, expressed in E. Coli.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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