Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - Ribosome Marker
  • Suitable for: WB, IP, IHC-P, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker
    See all RPS6 primary antibodies

  • Description

    Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - Ribosome Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RPS6. The exact sequence is proprietary.
    (Peptide available as ab175053)

  • Positive control

    • Human breast adenocarcinoma tissue; HeLa cell lysate, untreated or treated with Calyculin A.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP1338(2)Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
    Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
    All lanes : Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h. Whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 1 minute


    Blocking and diluting  buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

    ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
    Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

    Dot Blot analysis of -

    Lane 1: Rb (pS235) phospho peptide

    Lane 2: Rb (pS236) phospho peptide

    Lane 3: Rb (pS235 & pS236) phospho peptide

    Lane 4: Rb non-phospho peptide

    5% NFDM/TBST was used as the diluting and blocking buffer.

    ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

  • Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
    Western blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
    All lanes : Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/200000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate, treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)
    Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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