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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome

Anti-RPL22 antibody (ab77720)

Price and availability

284 784 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-RPL22 antibody (ab77720)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RPL22
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RPL22 antibody
    See all RPL22 primary antibodies
  • Description

    Rabbit polyclonal to RPL22
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human RPL22 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87822)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa; HEK293; Jurkat.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human RPL22 protein (ab167872)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab77720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Horse
Chicken
Pig
Zebrafish
Cynomolgus monkey
Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

  • Sequence similarities

    Belongs to the ribosomal protein L22e family.
  • Target information above from: UniProt accession P35268 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6146 Human
    • Entrez Gene: 100504863 Mouse
    • Entrez Gene: 19934 Mouse
    • Entrez Gene: 81768 Rat
    • Omim: 180474 Human
    • SwissProt: P35268 Human
    • SwissProt: P67984 Mouse
    • SwissProt: P47198 Rat
    • Unigene: 515329 Human
    • Unigene: 554762 Human
    • Unigene: 307846 Mouse
    • Unigene: 21738 Rat
    see all
  • Alternative names

    • 60S ribosomal protein L22 antibody
    • EAP antibody
    • EBER associated protein antibody
    • EBER-associated protein antibody
    • Epstein Barr encoded RNA associated protein antibody
    • Epstein Barr virus small RNA associated protein antibody
    • Epstein-Barr virus small RNA-associated protein antibody
    • HBP15 antibody
    • HBP15 L22 antibody
    • heparin binding protein 15 antibody
    • Heparin binding protein HBp15 antibody
    • Heparin-binding protein HBp15 antibody
    • ribosomal protein L22 antibody
    • RL22_HUMAN antibody
    • rpl22 antibody
    see all

Images

  • Western blot - Anti-RPL22 antibody (ab77720)
    Western blot - Anti-RPL22 antibody (ab77720)
    All lanes : Anti-RPL22 antibody (ab77720) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa
    Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab77720? Please let us know so that we can cite the reference in this datasheet.

    ab77720 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-RPL22 antibody (ab77720)
      Western blot - Anti-RPL22 antibody (ab77720)
      All lanes : Anti-RPL22 antibody (ab77720) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 15 kDa
      Observed band size: 15 kDa
      Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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