Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UBA52 antibody [EPR4547] (ab109230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4547] to UBA52
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-UBA52 antibody [EPR4547]
    See all UBA52 primary antibodies

  • Description

    Rabbit monoclonal [EPR4547] to UBA52

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta, Raji, fetal kidney, 293T and Jurkat lysates IHC-P: Human brain tissue IF/ICC: HepG2 cell line.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (ab109230)
    Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (ab109230)

    ab109230 stained HepG2 cells. The cells were 4%  formaldehdye fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109230 at 1/200) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96899 Dylight 488 goat anti-rabbit IgG used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-UBA52 antibody [EPR4547] (ab109230)
    Western blot - Anti-UBA52 antibody [EPR4547] (ab109230)
    All lanes : Anti-UBA52 antibody [EPR4547] (ab109230) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : Raji cell lysate
    Lane 3 : Fetal kidney lysate
    Lane 4 : 293T cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 15 kDa
    Observed band size: 10 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4547] (ab109230)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4547] (ab109230)

    Immunohistochemical analysis of UBA52 in paraffin embedded Human brain tissue, using ab109230 at a 1/500 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-UBA52 antibody [EPR4547] (ab109230)
    Anti-UBA52 antibody [EPR4547] (ab109230)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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