Anti-RPS6 (phospho S244 + S247) antibody (ab12865)
Key features and details
- Rabbit polyclonal to RPS6 (phospho S244 + S247)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RPS6 (phospho S244 + S247) antibody
See all RPS6 primary antibodies -
Description
Rabbit polyclonal to RPS6 (phospho S244 + S247) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide derived from the region of human RPS6 that contains serines 244 and 247 (unfortunately, the amino acid sequence is considered to be commercially sensitive)
-
Positive control
- HeLa +/- TNF-alpha or anisomycin
-
General notes
Centrifuge before opening to settle contents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Dulbecco's PBS without magnesium and calcium -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RPS6. The final product is generated by affinity chromatography using a RPS6-derived peptide that is phosphorylated at serines 244 and 247. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-RPS6 (phospho S244 + S247) antibody (ab12865)Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab12865 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to RPS6 [phospho S244 & phospho S247] blocks the signal, verifying the specificity of the antibody.
Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST
