Antibodies, Proteins, Kits and Reagents for Life Science

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (ab213165)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - BSA and Azide free
Suitable for: Dot blot, WB, IP, IHC-P
Reacts with: Human

Product name

Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free
See all RPS6 primary antibodies
Description

Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Dot blot, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human breast adenocarcinoma tissue; HeLa cell lysate, untreated or treated with Calyculin A. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145
General notes
ab213165 is the carrier-free version of ab80158 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab213165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1338(2)Y
Isotype

IgG
Research areas

Dot Blot - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (ab213165)

Dot Blot analysis of -

Lane 1: Rb (pS235) phospho peptide

Lane 2: Rb (pS236) phospho peptide

Lane 3: Rb (pS235 & pS236) phospho peptide

Lane 4: Rb non-phospho peptide

5% NFDM/TBST was used as the diluting and blocking buffer.

ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (ab213165)

ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (ab213165)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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