Anti-RPS6 antibody (ab59261)
Key features and details
- Rabbit polyclonal to RPS6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPS6 antibody
See all RPS6 primary antibodies -
Description
Rabbit polyclonal to RPS6 -
Host species
Rabbit -
Specificity
ab59261 detects endogenous levels of total RPS6 protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A synthesized non-phosphopeptide derived from human RPS6 around the phosphorylation site of serine 240 (R-A-SP-T-S).
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Positive control
- WB: Extracts from HeLa cells treated with TNF-a (20ng/ml, 2 min). IHC-P: Human skeletal muscle tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPS6 antibody (ab59261)All lanes : Anti-RPS6 antibody (ab59261) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated with TNF-a (20ng/ml, 2 min)
Lane 2 : Extracts from HeLa cells treated with TNF-a (20ng/ml, 2 min) with immunising peptide
Predicted band size: 30 kDa
Observed band size: 30 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody (ab59261)Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab59261 at a dilution of 1/50, in the presence (right) or absence (left) of immunising peptide. -
Western blot - Anti-RPS6 antibody (ab59261) Image courtesy of an anonymous Abreview.All lanes : Anti-RPS6 antibody (ab59261) at 1/500 dilution
Lane 1 : Purified 40S ribosomes
Lane 2 : Purified 60S ribosomes (negative control)
Lane 3 : Purified 80S ribosomes
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (whole molecule) conjugated to peroxidase at 1/5000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
