Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free
    See all Glucose Transporter GLUT4 primary antibodies

  • Description

    Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250949 is the carrier-free version of ab188317. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250949 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution

    All lanes : Human fetal heart whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab188317, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    This data was developed using ab188317, the same antibody clone in a different buffer formulation.Dot Blot analysis of Lane 1: Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2: Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab188317, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution

    Lane 1 : Untreated human fetal heart
    Lane 2 : Lambda phosphorylase treated human fetal heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab188317, the same antibody clone in a different buffer formulation.

     

  • Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)

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