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Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] - BSA and Azide free (ab238661)

Key features and details

  • Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] - BSA and Azide free
    See all Glucose Transporter GLUT4 primary antibodies

  • Description

    Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Cow, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab34088)

  • Positive control

    • WB: ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa. IHC-P: Human heart tissue. Flow cyt: NSO cells.

  • General notes

    ab238661 is a PBS-only buffer format of ab48547.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Flow Cytometry - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] - BSA and Azide free (ab238661)
    Flow Cytometry - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] - BSA and Azide free (ab238661)

    Overlay histogram showing NSO cells stained with ab48547 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab48547, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879 at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NSO cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was produced using the same antibody clone but in a different formulation; PBS and sodium azide (ab48547).

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