Anti-Glycerol kinase antibody (ab193521)
Key features and details
- Rabbit polyclonal to Glycerol kinase
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-Glycerol kinase antibody
See all Glycerol kinase primary antibodies -
Description
Rabbit polyclonal to Glycerol kinase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Escherichia coliBacteria
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Immunogen
Recombinant full length protein corresponding to Escherichia coli Glycerol kinase aa 1 to the C-terminus.
Database link: P0A6F3 -
Positive control
- WB: DH5a whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
>95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glycerol kinase antibody (ab193521)Anti-Glycerol kinase antibody (ab193521) at 2 µg/ml + DH5a whole cell lysate
Secondary
Polyclonal goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Glycerol kinase antibody (ab193521)All lanes : Anti-Glycerol kinase antibody (ab193521) at 3 µg/ml
Lane 1 : Rosseta bacteria lysate at 80 µg
Lane 2 : Rosseta bacteria lysate at 40 µg
Lane 3 : Rosseta bacteria lysate at 20 µg
Lane 4 : Rosseta bacteria lysate at 10 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/5000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
