Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glycerol kinase antibody [EPR6567] (ab126599)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6567] to Glycerol kinase
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Glycerol kinase antibody [EPR6567]
    See all Glycerol kinase primary antibodies

  • Description

    Rabbit monoclonal [EPR6567] to Glycerol kinase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Glycerol kinase aa 450-550. The exact sequence is proprietary.

  • Positive control

    • WB: Mouse adipose tissue lysate. Fetal liver, HEK-293T, Jurkat and HepG2 whole cell lysate (ab7900). Flow Cyt: Permeabilized HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    Western blot - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/500 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : GK knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab126599 observed at 61 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab126599 Anti-Glycerol kinase antibody [EPR6567] was shown to specifically react with Glycerol kinase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab267328 (knockout cell lysate ab257966) was used. Wild-type and Glycerol kinase knockout samples were subjected to SDS-PAGE. ab126599 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    Western blot - Anti-Glycerol kinase antibody [EPR6567] (ab126599) This image is courtesy of an anonymous Abreview
    All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution

    All lanes : Mouse adipose tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP-conjugated Donkey anti-rabbit polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Western blot - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    Western blot - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution

    Lane 1 : Fetal liver lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    Flow Cytometry - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    ab126599, at 1/100 dilution, staining Glycerol kinase in permeabilized HepG2 cells by Immunofluorescence (red). A rabbit IgG is used as a negative control (green).
  • OI-RD Scanning - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    OI-RD Scanning - Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Glycerol kinase antibody [EPR6567] (ab126599)
    Anti-Glycerol kinase antibody [EPR6567] (ab126599)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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