Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Granulin antibody [8H10] - BSA and Azide free (ab255790)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [8H10] to Granulin - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Granulin antibody [8H10] - BSA and Azide free
    See all Granulin primary antibodies

  • Description

    Rat monoclonal [8H10] to Granulin - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: J774A.1 whole cell lysate treated and not treated with PNGase F. Hepa1-6, 4T1, RAW264.7 and NIH/3T3 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255790 is the carrier-free version of ab252834. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255790 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Granulin antibody [8H10] - BSA and Azide free (ab255790)
    Western blot - Anti-Granulin antibody [8H10] - BSA and Azide free (ab255790)
    All lanes : Anti-Granulin antibody [8H10] (ab252834) at 1.14 µg/ml

    Lane 1 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
    Lane 2 : 4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate
    Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 64 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Granulin is a glycoprotein, the molecular weight observed is consistent with what has been described in the literature (PMID: 8496151).

    Exposure times: Lanes 1-2/4: 37 seconds; Lanes 3: 5 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252834).

  • Western blot - Anti-Granulin antibody [8H10] - BSA and Azide free (ab255790)
    Western blot - Anti-Granulin antibody [8H10] - BSA and Azide free (ab255790)
    All lanes : Anti-Granulin antibody [8H10] (ab252834) at 1.14 µg/ml

    Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate
    Lane 2 : J774A.1 treated with PNGase F whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 64 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 136 seconds


    Granulin is a glycoprotein, the molecular weight observed is consistent with what has been described in the literature (PMID: 8496151).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252834).

  • Anti-Granulin antibody [8H10] - BSA and Azide free (ab255790)
    Anti-Granulin antibody [8H10] - BSA and Azide free (ab255790)

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