Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4605] to Xanthine Oxidase
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Xanthine Oxidase antibody [EPR4605]
    See all Xanthine Oxidase primary antibodies

  • Description

    Rabbit monoclonal [EPR4605] to Xanthine Oxidase

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, Mouse and Rat liver tissue lysates, 293T cell lysates, Human milk IHC-P: Human liver tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    All lanes : Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/10000 dilution (Purified)

    Lane 1 : Mouse liver lysates
    Lane 2 : Rat liver lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Mouse brain lysates
    Lane 5 : Mouse lung lysates
    Lane 6 : Mouse kidney lysates
    Lane 7 : Mouse spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 146 kDa
    Observed band size: 146 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

    Xanthine Oxidase is abundantly expressed in liver and low in heart, brain and lung as what is described in PMID: 10462034 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Xanthine Oxidase with purified ab109235 at 1/100 dilution (2.3 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/5000 dilution (Purified) + Human milk lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 146 kDa
    Observed band size: 146 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    All lanes : Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver lysate
    Lane 2 : 293T lysate
    Lane 3 : Human milk lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 146 kDa



    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

    Immunohistochemical analysis of Xanthine Oxidase expression in paraffin-embedded Human liver tissue using ab109235 (unpurified) at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
    Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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