Alpha Ketoglutarate (alpha KG) Assay Kit (ab83431)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.01 nmol/well
Overview
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Product name
Alpha Ketoglutarate (alpha KG) Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
= 0.01 nmol/well -
Range
0.01 nmol/well - 10 nmol/well -
Assay time
0h 30m -
Product overview
Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG.
In the alpha KG assay protocol, alpha-ketoglutarate is transaminated with the generation of pyruvate, which is used to convert a nearly colorless probe to a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product.
Alpha Ketoglutarate assay protocol summary:
- add reaction mix to standard and sample wells
- incubate for 30 min
- analyze with a microplate reader -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests a-KG Assay Buffer WM 1 x 25ml a-KG Converting Enzyme (lyophilized) Purple 1 vial a-KG Development Enzyme Mix (lyophilized) Green 1 vial a-KG OxiRed Probe (in DMSO) Red 1 x 200µl a-KG Standard (10 µmol, lyophilized) Yellow 1 vial -
Research areas
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Relevance
Alpha ketoglutarate (Alpha-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing Alpha-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. Alpha-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. -
Alternative names
- Alpha KG
Images
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Functional Studies - Alpha Ketoglutarate Assay Kit (ab83431)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Alpha Ketoglutarate Assay Kit (ab83431)Standard curve: mean of duplicates (+/- SD) with background reads subtracted