Anti-GANAB antibody (ab96757)
Key features and details
- Rabbit polyclonal to GANAB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GANAB antibody
See all GANAB primary antibodies -
Description
Rabbit polyclonal to GANAB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human GANAB aa 97-261.
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and NIH-3T3 whole cell lysates; HeLa cells; SW480 xenograft; human breasr carcinoma tissue.
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General notes
This product was previously labelled as alpha Glucosidase II
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Mehanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells staibed forGANAB using ab96757 at1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-Alpha tubulin antibody (red).
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All lanes : Anti-GANAB antibody (ab96757) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : GANAB knockout HeLa cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG HRP
Predicted band size: 107 kDa
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Paraffin-embedded human breast carcinoma tissue stained for GANAB using ab96757 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Anti-GANAB antibody (ab96757) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 107 kDa
7.5% SDS PAGE -
All lanes : Anti-GANAB antibody (ab96757) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : Molt-4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 107 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of GANAB in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution.
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Immunofluorescence analysis of GANAB in methanol-fixed HeLa cells, using ab96757 at 1/200 dilution (1) and costained with Hoechst 33342 (2).