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Signal Transduction Metabolism Energy Metabolism

Anti-GANAB antibody (ab96757)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-GANAB antibody (ab96757)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GANAB
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GANAB antibody
    See all GANAB primary antibodies
  • Description

    Rabbit polyclonal to GANAB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human GANAB aa 97-261.

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and NIH-3T3 whole cell lysates; HeLa cells; SW480 xenograft; human breasr carcinoma tissue.
  • General notes

     This product was previously labelled as alpha Glucosidase II

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (ab96757)
    Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (ab96757)

    Mehanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells staibed forGANAB using ab96757 at1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-Alpha tubulin antibody (red).

  • Western blot - Anti-GANAB antibody (ab96757)
    Western blot - Anti-GANAB antibody (ab96757)
    All lanes : Anti-GANAB antibody (ab96757) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : GANAB knockout HeLa cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG HRP

    Predicted band size: 107 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (ab96757)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (ab96757)

    Paraffin-embedded human breast carcinoma tissue stained for GANAB using ab96757 at 1/500 dilution in immunohistochemical analysis.  

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-GANAB antibody (ab96757)
    Western blot - Anti-GANAB antibody (ab96757)
    Anti-GANAB antibody (ab96757) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • Western blot - Anti-GANAB antibody (ab96757)
    Western blot - Anti-GANAB antibody (ab96757)
    All lanes : Anti-GANAB antibody (ab96757) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : HepG2 whole cell lysate
    Lane 6 : Molt-4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (ab96757)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (ab96757)

    Immunohistochemical analysis of GANAB in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (ab96757)
    Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (ab96757)

    Immunofluorescence analysis of GANAB in methanol-fixed HeLa cells, using ab96757 at 1/200 dilution (1) and costained with Hoechst 33342 (2).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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