Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GANAB antibody [EPR12377(B)] (ab179805)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12377(B)] to GANAB
Suitable for: Flow Cyt, IP, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-GANAB antibody [EPR12377(B)]
See all GANAB primary antibodies
Description

Rabbit monoclonal [EPR12377(B)] to GANAB
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human GANAB aa 650-750 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14697
Positive control
- Human placenta, A431, HepG2, HeLa, PC-12 and NIH3T3 lysates; HeLa cells.
General notes
This product was previously labelled as alpha Glucosidase II

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12377(B)
Isotype

IgG
Research areas

Western blot - Anti-GANAB antibody [EPR12377(B)] (ab179805)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GANAB knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: 1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab179805 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab179805 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab179805 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] (ab179805)

Western blot analysis on immunoprecipitation pellet from A431 cell lysate using ab179805.
Flow Cytometry - Anti-GANAB antibody [EPR12377(B)] (ab179805)

Flow cytometric analysis of permeabilized HeLa cells labeling GANAB with ab179805 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
Western blot - Anti-GANAB antibody [EPR12377(B)] (ab179805)

All lanes : Anti-GANAB antibody [EPR12377(B)] (ab179805) at 1/1000 dilution

Lane 1 : Human placenta lysate
Lane 2 : A431 lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate
Lane 5 : PC-12 lysate
Lane 6 : NIH3T3 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 107 kDa
Anti-GANAB antibody [EPR12377(B)] (ab179805)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com