Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GANAB antibody [EPR12377(B)] (ab179805)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12377(B)] to GANAB
  • Suitable for: Flow Cyt, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GANAB antibody [EPR12377(B)]
    See all GANAB primary antibodies

  • Description

    Rabbit monoclonal [EPR12377(B)] to GANAB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GANAB aa 650-750 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q14697

  • Positive control

    • Human placenta, A431, HepG2, HeLa, PC-12 and NIH3T3 lysates; HeLa cells.

  • General notes

     This product was previously labelled as alpha Glucosidase II

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GANAB antibody [EPR12377(B)] (ab179805)
    Western blot - Anti-GANAB antibody [EPR12377(B)] (ab179805)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GANAB knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: 1 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab179805 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab179805 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab179805 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] (ab179805)
    Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] (ab179805)

    Western blot analysis on immunoprecipitation pellet from A431 cell lysate using ab179805.

  • Flow Cytometry - Anti-GANAB antibody [EPR12377(B)] (ab179805)
    Flow Cytometry - Anti-GANAB antibody [EPR12377(B)] (ab179805)

    Flow cytometric analysis of permeabilized HeLa cells labeling GANAB with ab179805 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

  • Western blot - Anti-GANAB antibody [EPR12377(B)] (ab179805)
    Western blot - Anti-GANAB antibody [EPR12377(B)] (ab179805)
    All lanes : Anti-GANAB antibody [EPR12377(B)] (ab179805) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : A431 lysate
    Lane 3 : HepG2 lysate
    Lane 4 : HeLa lysate
    Lane 5 : PC-12 lysate
    Lane 6 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 107 kDa

  • Anti-GANAB antibody [EPR12377(B)] (ab179805)
    Anti-GANAB antibody [EPR12377(B)] (ab179805)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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