Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GANAB antibody [EPR12376] (ab176349)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12376] to GANAB
Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-GANAB antibody [EPR12376]
See all GANAB primary antibodies
Description

Rabbit monoclonal [EPR12376] to GANAB
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human GANAB aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14697
Positive control
- HeLa, Human placenta, A431 and HepG2 lysates. Paraffin-embedded Human gastric carcinoma tissue, paraffin-embedded Human placenta tissue, permeabilized Hela cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as alpha Glucosidase II

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12376
Isotype

IgG
Research areas

Western blot - Anti-GANAB antibody [EPR12376] (ab176349)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GANAB knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab176349 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab176349 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab176349 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-GANAB antibody [EPR12376] (ab176349)

Flow cytometric analysis of permeabilized Hela cells labeling GANAB with ab176349 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody [EPR12376] (ab176349)

Immunofluorescent staining of HeLa cells labeling GANAB with ab176349 at 1/50 dilution (red). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GANAB with ab176349 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)

Immunohistochemical analysis of paraffin-embedded Humangastric carcinoma tissue labeling GANAB with ab176349 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-GANAB antibody [EPR12376] (ab176349)

All lanes : Anti-GANAB antibody [EPR12376] (ab176349) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Human placenta lysate
Lane 3 : A431 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 107 kDa
Anti-GANAB antibody [EPR12376] (ab176349)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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