Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GANAB antibody [EPR12376] (ab176349)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12376] to GANAB
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GANAB antibody [EPR12376]
    See all GANAB primary antibodies

  • Description

    Rabbit monoclonal [EPR12376] to GANAB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GANAB aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q14697

  • Positive control

    • HeLa, Human placenta, A431 and HepG2 lysates. Paraffin-embedded Human gastric carcinoma tissue, paraffin-embedded Human placenta tissue, permeabilized Hela cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as alpha Glucosidase II

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GANAB antibody [EPR12376] (ab176349)
    Western blot - Anti-GANAB antibody [EPR12376] (ab176349)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GANAB knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab176349 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab176349 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab176349 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-GANAB antibody [EPR12376] (ab176349)
    Flow Cytometry - Anti-GANAB antibody [EPR12376] (ab176349)

    Flow cytometric analysis of permeabilized Hela cells labeling GANAB with ab176349 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody [EPR12376] (ab176349)
    Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody [EPR12376] (ab176349)

    Immunofluorescent staining of HeLa cells labeling GANAB with ab176349 at 1/50 dilution (red). DAPI nuclear staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GANAB with ab176349 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody [EPR12376] (ab176349)

    Immunohistochemical analysis of paraffin-embedded Humangastric carcinoma tissue labeling GANAB with ab176349 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GANAB antibody [EPR12376] (ab176349)
    Western blot - Anti-GANAB antibody [EPR12376] (ab176349)
    All lanes : Anti-GANAB antibody [EPR12376] (ab176349) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human placenta lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 107 kDa

  • Anti-GANAB antibody [EPR12376] (ab176349)
    Anti-GANAB antibody [EPR12376] (ab176349)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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