ENO1 Assay Kit (alpha Enolase, Human) (ab117994)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture extracts, Tissue Extracts
- Sensitivity: 125 ng/ml
Overview
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Product name
ENO1 Assay Kit (alpha Enolase, Human) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 9 9% Inter-assay Sample n Mean SD CV% Cell extract 4 11% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
125 ng/ml -
Range
125 ng/ml - 2000 ng/ml -
Recovery
90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 63 % - % Serum 140 % - % -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Product overview
ENO1 Assay Kit (alpha Enolase, Human) ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 20X Buffer 1 x 20ml Base Buffer 1 x 24ml Extraction Buffer (ab260490) 1 x 15ml LDH (Lactate Dehydrogenase) 1 x 187.5 units Microplate 96 antibody coated wells in 12 strips 1 unit PK (Pyruvate Kinase) 1 x 187.5 units Reagent Mix 1 x 1ml -
Research areas
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Database links
- Entrez Gene: 2023 Human
- Entrez Gene: 2023 Human
- SwissProt: P06733 Human
Images
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Functional Studies - ENO1 Human Activity Assay Kit (ab117994)Scheme of reactions used in this kit. Abbreviations are as follows: enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).
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Immunocytochemistry/ Immunofluorescence - ENO1 Human Activity Assay Kit (ab117994)Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994. -
Functional Studies - ENO1 Human Activity Assay Kit (ab117994)Example of dilution series prepared from HepG2 cells extract. -
Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
