Enolase Assay Kit (ab241024)
Key features and details
- Detection method: Colorimetric/Fluorometric
- Sample type: Adherent cells, Cell culture supernatant, Suspension cells, Tissue
Overview
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Product name
Enolase Assay Kit
See all ENO1 kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Tissue, Adherent cells, Suspension cells -
Product overview
In the Enolase Assay Kit (ab241024) enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with the probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm).
This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Enolase Assay Buffer 1 x 25ml Enolase Converter (Lyophilized) 1 vial Enolase Developer (Lyophilized) 1 vial Enolase Positive Control (Lyophilized) 1 vial Enolase Substrate Mix (Lyophilized) 1 vial H2O2 Standard (0.88 M) 1 x 0.1ml Probe (in DMSO) 1 x 0.2ml -
Research areas
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Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Post-translational
modificationsISGylated. -
Cellular localization
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. - Information by UniProt
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Alternative names
- 2 phospho D glycerate hydro lyase
- 2-phospho-D-glycerate hydro-lyase
- Alpha enolase
see all
Images
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H2O2 Standard Curve for Colorimetric Assay.
H2O2 Standard Curve for Colorimetric Assay.
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H2O2 Standard Curve for Fluorometric Assay.H2O2 Standard Curve for Fluorometric Assay.
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Enolase Activity.Enolase Activity in rat muscle lysate (1 µg), HeLa lysate (5 µg) and positive control. Assays were performed following the kit protocol.
