Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDK2 antibody [EPR1948Y] (ab68164)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1948Y] to PDK2
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDK2 antibody [EPR1948Y]
    See all PDK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR1948Y] to PDK2

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Rat
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PDK2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q15119

  • Positive control

    • WB: Mouse, rat and human heart lysate, rat skeletal lysate, HAPI and SHSY5Y cell lysates; IP: Rat heart lysate; IHC-P: Human bladder carcinoma and cardiac muscle.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Western blot - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Anti-PDK2 antibody [EPR1948Y] (ab68164) at 1/200 dilution (Purified) + Human heart lysate

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling PDK2 with Purified ab68164 at 1/1000 dilution (0.12 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Western blot - Anti-PDK2 antibody [EPR1948Y] (ab68164)

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: PDK2 knockout HAP1 cell lysate (40 µg)
    Lane 3: SH-SY5Y cell lysate (40 ug)
    Lanes 1 - 3: Merged signal (red and green). Green - ab68164 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab68164 was shown to specifically react with PDK2 in wild-type HAP1 cells. No band was observed when PDK2 knockout samples were examined. Wild-type and PDK2 knockout samples were subjected to SDS-PAGE. Ab68164 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4oC. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) ab216773 and IRDye® 680 Goat anti-Mouse IgG (H + L) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Immunoprecipitation - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Purified ab68164 at 1/20 dilution (0.6µg) immunoprecipitating PDK2 in Rat heart lysate.
    Lane 1 (input): Rat heart lysate 10µg
    Lane 2 (+): ab68164 + Rat heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68164 in Rat heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 48 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling PDK2 with Purified ab68164 at 1/1000 dilution (0.12 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Western blot - Anti-PDK2 antibody [EPR1948Y] (ab68164)
    All lanes : Anti-PDK2 antibody [EPR1948Y] (ab68164) at 1/1000 dilution (Purified)

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat skeletal muscle lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 46 kDa

  • Anti-PDK2 antibody [EPR1948Y] (ab68164)
    Anti-PDK2 antibody [EPR1948Y] (ab68164)

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