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Anti-PDK2 antibody [3F2D7] (ab201964)

Key features and details

  • Mouse monoclonal [3F2D7] to PDK2
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Human, African green monkey
  • Isotype: IgG2b

Overview

  • Product name

    Anti-PDK2 antibody [3F2D7]
    See all PDK2 primary antibodies

  • Description

    Mouse monoclonal [3F2D7] to PDK2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human PDK2 aa 150 to the C-terminus. Expressed in E. Coli.
    Database link: Q15119

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • A431 cells; Human recombinant PDK2 (aa178-404) protein; Human recombinant PDK2 (aa178-404)-hIgGFc transfected HEK293 cell lysate; Jurkat , C6), COS7, K562 and A431 cell lysates.

Properties

Images

  • Western blot - Anti-PDK2 antibody [3F2D7] (ab201964)
    Western blot - Anti-PDK2 antibody [3F2D7] (ab201964)

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: PDK2 knockout HAP1 cell lysate (40 µg)
    Lane 3: SHSY5Y cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab201964 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab201964 was shown to recognize PDK2 when PDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and PDK2 knockout samples were subjected to SDS-PAGE.  Ab201964 and ab181602 (loading control to GAPDH) were diluted at 1/2000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) ab216772 and IRDye® 680 Goat anti-Rabbit IgG (H + L) ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-PDK2 antibody [3F2D7] (ab201964)
    Flow Cytometry - Anti-PDK2 antibody [3F2D7] (ab201964)

    Flow cytometric analysis of A431 cells labeling PDK2 with ab201964 at 1/200 dilution (green). Negative control (red).

  • Western blot - Anti-PDK2 antibody [3F2D7] (ab201964)
    Western blot - Anti-PDK2 antibody [3F2D7] (ab201964)
    All lanes : Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : A431 cell lysate

    Predicted band size: 46 kDa

  • Western blot - Anti-PDK2 antibody [3F2D7] (ab201964)
    Western blot - Anti-PDK2 antibody [3F2D7] (ab201964)
    All lanes : Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Human recombinant PDK2 (aa178-404)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 46 kDa

  • Western blot - Anti-PDK2 antibody [3F2D7] (ab201964)
    Western blot - Anti-PDK2 antibody [3F2D7] (ab201964)
    Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution + Human recombinant PDK2 (aa178-404) protein

    Predicted band size: 46 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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