Anti-ATXN1L antibody (ab241958)
Key features and details
- Rabbit polyclonal to ATXN1L
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATXN1L antibody
See all ATXN1L primary antibodies -
Description
Rabbit polyclonal to ATXN1L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human -
Immunogen
Synthetic peptide within Human ATXN1L aa 250-300. The exact sequence is proprietary.
Database link: P0C7T5 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
ab241958 has not performed satisfactorily when used for WB of ATXN1L in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ATXN1L.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab241958 was affinity purified using an epitope specific to ATXN1L immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ATXN1L was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241958 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241958 at 0.4 µg/ml.
Lane 1: ab241958 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.