Immunohistochemical analysis of paraffin-embedded human lymphoid tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.

Immunohistochemical analysis of paraffin-embedded human testicle tumor tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.

ab47720 at 1/1000 dilution staining Inhibin alpha in human Hela cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary show green staining in image while actin filaments have been labelled red with DY-554 phalloidin. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.

Overlay histogram showing HeLa cells stained with ab47720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab47720, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.