Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vasopressin antibody [EPR20602] (ab213708)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20602] to Vasopressin
  • Suitable for: WB, IP, Flow Cyt, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Vasopressin antibody [EPR20602]
    See all Vasopressin primary antibodies

  • Description

    Rabbit monoclonal [EPR20602] to Vasopressin

  • Host species

    Rabbit

  • Specificity

    In WB, FC and IP we detected Vasopressin only in overexpressing cells.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Recombinant fragment
    IP
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: Mouse and rat hypothalamus tissues. IHC-Fr: Mouse and rat brain paraventricular nucleus. Flow Cyt: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®. IP: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)
    Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)
    All lanes : Anti-Vasopressin antibody [EPR20602] (ab213708) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

    Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of mouse paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of rat paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
    Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling Vasopressin with ab213708  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on mouse brain tissue section is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)
    Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling Vasopressin with ab213708  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on rat brain tissue section is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-Vasopressin antibody [EPR20602] (ab213708)
    Flow Cytometry - Anti-Vasopressin antibody [EPR20602] (ab213708)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag® cell line labeling Vasopressin with ab213708 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Co-stained with Alexa Fluor® 647 conjugated Myc-tag (Y-axis). Only Myc (+) population showed Vasopressin positive staining.

  • Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)
    Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)

    Vasopressin was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate with ab213708 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate 10 µg (Input). 

    Lane 2: ab213708 IP in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213708 in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-Vasopressin antibody [EPR20602] (ab213708)
    Anti-Vasopressin antibody [EPR20602] (ab213708)

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